"Christmas Eve/Sarajevo 12/24" is an instrumental medley of "God Rest Ye Merry, Gentlemen" and "Shchedryk", first released on the Savatage album Dead Winter Dead in 1995 as "Christmas Eve (Sarajevo 12/24)." It was re-released by the Trans-Siberian Orchestra, a side project of several Savatage members, on their 1996 debut album Christmas Eve and Other Stories. The piece describes a lone cello player (based on Vedran Smailović) playing a forgotten Christmas carol in war-torn Sarajevo.
Both of the tunes used in "Christmas Eve/Sarajevo 12/24" were in the public domain in 1995: "Shchedryk" was released in 1918 (although the English lyrics to "Carol of the Bells," dating to 1936, were still under copyright and were not included in the recording), while "God Rest Ye Merry, Gentlemen" dated back several centuries.
The causes of different sensitivity of mouse LS lymphosarcoma and its resistant RLS variant to cyclophosphamide were studied. Division of LS and RLS cells stops in the G2/M phase 24 h after cyclophosphamide treatment, but this stop lasts for more than 48 h in LS cells and less than 24 h in RLS cells. DNA fragmentation, a marker of apoptosis, is observed only in LS cells starting from 24 h after cyclophosphamide treatment. LS and RLS strains do not differ by the expression of bcl-2, bcl-6, bax, bad, mdr1a, mdr1b genes and P-glycoprotein protein. The strains differ by transport activity of P-glycoprotein, tested by SYTO 16 substrate release from cells: activity of P-glycoprotein in RLS cells was 2-fold higher than in LS cells. Presumably, the resistance of RLS tumor to cyclophosphamide-induced apoptosis is a result of inhibition of the apoptotic cascade by P-glycoprotein which is functionally more active in these cells than in LS cells. 2b1af7f3a8